Fig. 9. Gel image of Genomic Cleavage Detection Assay of luteal cells transfected with components of CRISPR/Cas9 system for knocking out TSP1 using Lipofectamine(r) 2000. Lane 1: negative control sample for TSP1 gene. Lane 2: Showing parent and both the cleaved bands. The above samples were PCR amplified using the same set of primers flanking the region of interest. After re-annealing, samples were treated with and without Detection Enzyme and run on a 2% E-Gel(r) EX Gel. Lane M: 100 bp DNA